Detecting RNA base methylations in single cells by in situ hybridization
oleh: Rohan T. Ranasinghe, Martin R. Challand, Kristina A. Ganzinger, Benjamin W. Lewis, Charlotte Softley, Wolfgang H. Schmied, Mathew H. Horrocks, Nadia Shivji, Jason W. Chin, James Spencer, David Klenerman
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2018-02-01 |
Deskripsi
Methylated RNA bases influence many life processes, but current detection methods lack the ability to detect individual methylations in single cells. Here, the authors use fluorescence hybridization probes sensitive to methylation to detect specific epitranscriptomic modifications at the single-cell level.