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Simple sequence repeat (SSR) markers are powerful tools for the determination ofrelationships between genotypes and genetic diversity because of advantages such as high polymorphism, codominance nature, multiallelism and distribution on encoding and non-encoding regions, This study investigated the transferability and polymorphism of 102 barley SSR markers in 41 bread and durum wheat and triticale genotypes. A total of 50 pair primers were transferable,and 40 pair primers producing 118 alleles(onaverage 2.95 allelesper locus) were polymorphic. The Polymorphism Information Content and genetic diversity for these markers were estimated to be 29% and 33%, respectively. The average of Shannon and Nei indices for the studied genotypes were 34% and 23%, respectively. Cluster analysis, using a Minimum Evolution algorithm and P-distance coefficient, assigned the genotypes into five groups. In principal coordinates analysis, the first principal component explained 74.69% of the total molecular variation. Analysis of molecular variance based on the genome structure of the genotypes using molecular data revealed a high level of variation within groups. In conclusion, this study has identified a set of 50 polymorphic SSR markers of barley as being useful for diversityanalysis of common wheat, durum wheat, and triticale. These microsatellites could be used for diversity analysis, molecular mapping/QTL analysis, molecular marker-assisted selection, and comparative genome analysis.