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Animal production is highly affected due to ticks and diseases caused by ticks. Tick control is critical for the economic well-being of the animal industry. This study focused on anti-tick properties of the Neem, Azadirachta indica extracts against Rhipicephalus sanguineus in vitro conditions. R. sanguineus exhibited a high (P < 0.05) dose revolting reaction to A. indica. There were no significant differences in reaction magnanimity between both sexes. The essence also prominently (p < 0.05) retarded the shedding of 60 % of R. sanguineus engorged nymphs. It resulted in that mortality percentage increasing with an increase in the dose of A. indica. At 20 % of A. indica, 87.76 % of tick mortality was recorded, while 53.23 % at 15% concentration. Probit analysis showed a repellent EC50 of A. indica for male and female ticks was 4.87, and 4.87 mg/mL, respectively. The findings advocate to A. indica is potent enough to use for anti-tick action. Further studies are needed to test Neem extract on other developmental stages i.e. eggs, nymphs, and larvae of tick.