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The present study measured the biomass and secondary production of zooplanktonic populations of Berlengas Biosphere Reserve of UNESCO (BBR), a Marine Protected Area (MPA) located on the west coast of Portugal. Sampling campaigns were performed between June 2010 and August 2012. Samples were collected on a Bongo net with 200µm and 500µm meshes at 7 stations around Berlengas’ Archipelago. From the 15 sampling moments, 11 occurred in upwelling-favourable conditions. The data obtained on this study revealed that copepoda were 62% of zooplanktonic biomass in samples from 500µm mesh and 23% in samples from 200µm mesh. Higher values of copepod mean biomass (carbon content - mgC.m-3) were found during spring and summer months. Secondary production rates were also higher during spring and summer months with maximum mean values on August 2012 (0,053 ± 0,126 mgC.m-3d-1). Higher levels of phytoplankton biomass (chlorophyll a) and secondary production rates occurred during spring and summer, when upwelling-favourable conditions were observed.