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The demand for using vegetarian foods more and more increase nowadays with focusing to the veganism that mean don’t contain any ingredients have origin from animals that is an important feature in the processing and production of vegetarian foods. For this purpose, we check whether have or not presence of animal ingredients in vegetarian food. PCR assay that are specific to detect the presence of ingredients animal were designed basing on 16S rDNA gene of the mitochondrial DNA genome by primer TP1 and TP2. Oligonucleotide primers that are universal and specific for 16S rDNA gene in most of animals. As the results, assay was initially successfully established for detecting animal-origin components in vegetarian foods. We experimentally tested and detected animal ingredients in 11 samples vegetarian food from the market and vegetarian food companies, as the results, 4/11 samples (counting for 36.36%) contain the animal ingredients. In addition, we conducted sequencing and indentified successfully this ingredient originating from Sus scrofa and Gallus gallus. According to this sequencing result that are completely accordant, we affirm primer-specific amplification in this study can be apply to experiment on the large number of samples in the reality.