Find in Library

The annual species <i>Dasypyrum villosum</i> possesses several potentially valuable genes for the improvement of common wheat. Previously, we identified a new stripe rust-resistant line, the Chinese Spring (CS)–<i>D. villosum</i> 3V#3 (3D) substitution line (named CD-3), and mapped its potential rust resistance gene (designated as <i>YrCD-3</i>) on the 3V#3 chromosome originating from <i>D. villosum</i>. The objective of the present study was to further narrow down the <i>YrCD-3</i> locus to a physical region and develop wheat-3V#3 introgression lines with strong stripe rust resistance. By treating CD-3 seeds with ⁶⁰Co γ-irradiation, two CS-3V#3 translocation lines, T3V#3S.3DL and T3DS.3V#3L (termed 22-12 and 24-20, respectively), were identified from the M₄ generation through a combination of non-denaturing fluorescence in situ hybridization (ND-FISH) and functional molecular markers. Stripe rust resistance tests showed that the line 22-12 exhibited strong stripe rust resistance similarly to CD-3, whereas 24-20 was susceptible to stripe rust similarly to CS, indicating that <i>YrCD-3</i> is located on the short arm of 3V#3. The line 22-12 can potentially be used for further wheat improvement. Additionally, to trace 3V#3 in the wheat genetic background, we produced 30 3V#3-specific sequence tag (EST) markers, among which, 11 markers could identify 3V#3S. These markers could be valuable in fine-mapping <i>YrCD-3</i>.