CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells
oleh: Mitchell G. Kluesner, Walker S. Lahr, Cara-lin Lonetree, Branden A. Smeester, Xiaohong Qiu, Nicholas J. Slipek, Patricia N. Claudio Vázquez, Samuel P. Pitzen, Emily J. Pomeroy, Madison J. Vignes, Samantha C. Lee, Samuel P. Bingea, Aneesha A. Andrew, Beau R. Webber, Branden S. Moriarity
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2021-04-01 |
Deskripsi
Base editors can inactivate splice sites or introduce stop codons into a gene sequence. Here the authors present SpliceR to design, rank, and test sgRNAs for efficient gene disruption in T cells.