From SOMAmer-based biomarker discovery to diagnostic and clinical applications: a SOMAmer-based, streamlined multiplex proteomic assay.
oleh: Stephan Kraemer, Jonathan D Vaught, Christopher Bock, Larry Gold, Evaldas Katilius, Tracy R Keeney, Nancy Kim, Nicholas A Saccomano, Sheri K Wilcox, Dom Zichi, Glenn M Sanders
| Format: | Article |
|---|---|
| Diterbitkan: | Public Library of Science (PLoS) 2011-01-01 |
Deskripsi
Recently, we reported a SOMAmer-based, highly multiplexed assay for the purpose of biomarker identification. To enable seamless transition from highly multiplexed biomarker discovery assays to a format suitable and convenient for diagnostic and life-science applications, we developed a streamlined, plate-based version of the assay. The plate-based version of the assay is robust, sensitive (sub-picomolar), rapid, can be highly multiplexed (upwards of 60 analytes), and fully automated. We demonstrate that quantification by microarray-based hybridization, Luminex bead-based methods, and qPCR are each compatible with our platform, further expanding the breadth of proteomic applications for a wide user community.