Barcode-free next-generation sequencing error validation for ultra-rare variant detection
oleh: Huiran Yeom, Yonghee Lee, Taehoon Ryu, Jinsung Noh, Amos Chungwon Lee, Han-Byoel Lee, Eunji Kang, Seo Woo Song, Sunghoon Kwon
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2019-02-01 |
Deskripsi
Next generation sequencing has difficulty in detecting rare-frequency variants due to high sequencing errors. Here the authors present a barcode-free error validation method that physically extracts erroneous reads to identify true variants.