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Context: Diarrhoea is the most prevalent cause of morbidity and mortality among children aged under five years. To address this, an education programme is administered. Aim: To assess the effectiveness of a structured educational programme over knowledge of ORS. Methods and Material: 60 mothers aged (18–35 years) of under five-year children were enrolled in this experimental quantitative study by non-probability purposive sampling method. A pre-test and post-test questionnaire administered to a group of mothers. Statistical analysis Paired t-test & Chi-Square test were applied whereas appliable. Results: This study showed a mean improvement of 7.36 (36.85%) in the score of pre-test and post-test as the mean score of the pre-test is 9.37 ± 4.96, and the mean score of post-test is 16.73 ± 2.97, respectively, which reveals that there is a significant improvement in the knowledge of the mothers after the structured educational program on ORS therapy. Conclusion: It is drawn from this study that a structured teaching programme was effective significantly in improving mothers' knowledge regarding oral rehydration therapy.