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Molecular characterisation of six Riemerella anatipestifer isolates were carried out by enterobacterial repetitive intergenic consenses polymerase chain reaction (ERICPCR). On analyzing the ERIC-PCR profile, ten to twelve bands per isolate were observed, with a size of approximately 150 bp to 1000 bp. The distance matrices were calculated using the GelQuest - DNA fingerprint analysis software and it was used to construct the phylogenetic relationships among the isolates by the neighbour-joining method. The isolate RA2 was distinct from others. The isolates RA1, RA3, RA4, RA5 having same band pattern approximately at 700 bp were grouped together and separated from RA6. The existence of genetic heterogeneity between the isolates analysed were noticed.