Cell culture NAIL-MS allows insight into human tRNA and rRNA modification dynamics in vivo
oleh: Matthias Heiss, Felix Hagelskamp, Virginie Marchand, Yuri Motorin, Stefanie Kellner
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2021-01-01 |
Deskripsi
Post transcriptional modification of RNAs represents an important layer of gene regulation. Here the authors describe NAIL-MS—a method for monoisotopic RNA labeling in cell culture—demonstrating its capabilities by analyzing the modification kinetics of total tRNA, 18S rRNA and tRNAPhe as models.