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Basic leucine zipper (bZIP) transcription factors (TFs) are one of the largest families involved in plant physiological processes such as biotic and abiotic responses, growth, and development, etc. In this study, 66 members of the bZIP family were identified in <i>Bletilla striata</i>, which were divided into 10 groups based on their phylogenetic relationships with <i>AtbZIPs</i>. A structural analysis of <i>BsbZIPs</i> revealed significant intron–exon differences among <i>BsbZIPs</i>. A total of 63 bZIP genes were distributed across 16 chromosomes in <i>B. striata</i>. The tissue-specific and germination stage expression patterns of <i>BsbZIPs</i> were based on RNA-seq. Stress-responsive expression analysis revealed that partial <i>BsbZIPs</i> were highly expressed under low temperatures, wounding, oxidative stress, and GA treatments. Furthermore, subcellular localization studies indicated that <i>BsbZIP13</i> was localized in the nucleus. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays suggested that <i>BsbZIP13</i> could interact with multiple <i>BsSnRK2s</i>. The results of this study provide insightful data regarding bZIP TF as one of the stress response regulators in <i>B. striata</i>, while providing a theoretical basis for transgenic and functional studies of the bZIP gene family in <i>B. striata</i>.