Highly Sensitive Electrochemical Aptasensor for Detecting the VEGF<sub>165</sub> Tumor Marker with PANI/CNT Nanocomposites

oleh: Yunjeong Park, Min-Sung Hong, Woo-Hyuk Lee, Jung-Gu Kim, Kyunghoon Kim

Format: Article
Diterbitkan: MDPI AG 2021-04-01

Deskripsi

Sensing targeted tumor markers with high sensitivity provides vital information for the fast diagnosis and treatment of cancer patients. A vascular endothelial growth factor (VEGF<sub>165</sub>) have recently emerged as a promising biomarker of tumor cells. The electrochemical aptasensor is a promising tool for detecting VEGF<sub>165</sub> because of its advantages such as a low cost and quantitative analysis. To produce a sensitive and stable sensor electrode, nanocomposites based on polyaniline (PANI) and carbon nanotube (CNT) have potential, as they provide for easy fabrication, simple synthesis, have a large surface area, and are suitable in biological environments. Here, a label-free electrochemical aptasensor based on nanocomposites of CNT and PANI was prepared for detecting VEGF<sub>165</sub> as a tumor marker. The nanocomposite was assembled with immobilized VEGF<sub>165</sub> aptamer as a highly sensitive VEGF<sub>165</sub> sensor. It exhibited stable and wide linear detection ranges from 0.5 pg/mL to 1 μg/mL, with a limit of detection of 0.4 pg/mL because of the complementary effect of PANI/CNT. The fabricated aptasensor also exhibited good stability in biological conditions, selectivity, and reproducibility after several measurement times after the dissociation process. Thus, it could be applied for the non-invasive determination of VEGF, in biological fluid diagnosis kits, or in an aptamer-based biosensor platform in the near future.