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Background and Objective: The widespread use of electromagnetic waves in human life has required the scientific research in the effects of these waves on the health of living beings. Due to the widespread use of these waves and the contradictory results in various researches, more comprehensive investigations are needed in this field. Therefore, the aim of this study was to evaluate the effect of 100µT magnetic fields on blood sample parameters such as: Cortisol, Blood Sugar, Triiodothyronine, and Thyroxin hormones in rat. Materials and Methods: The study was conducted on 24 male rats (7-8 weeks old). The animals were randomly divided into two equal groups: experimental group and control group. The experimental group was exposed in a 100 micro-Tesla magnetic field, and control group was exposed in the quasi field in the same conditions. At the end of the experiment, all rats were scarifies, and blood samples were drawn. Results: Statistical analysis indicated that there were significant differences between both experimental and control groups on Cortisol (P<0.001), Blood Sugar (P<0.001) and Triiodothyronine (P<0.026). There was no significant effect on Thyroxin. Conclusion: The results of this study indicated significant decreases in the level of Cortisol and blood glucose and an increase in the level of Triiodothyronine hormones due to the magnetic fields. Since Cortisol is a hormone that can affect blood pressure and blood sugar, these variations can effect on human health.