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<p>Abstract</p> <p><it>Mycoplasma alkalescens </it>is an arginine-metabolizing mycoplasma, which has been found in association with mastitis and arthritis in cattle. Routine bacteriological examination of 17 bronchoalveolar lavage samples from calves with pneumonia in a single herd in Denmark, identified <it>M. alkalescens </it>in eight samples. The organism was found as a sole bacterilogical findings in five of the samples as well as in combination with <it>Mannheimia haemolytica</it>, <it>Haemophilus somni </it>and <it>Salmonella </it>Dublin. This is the first report of isolation of <it>M. alkalescens </it>in Denmark.</p>