Pooled image-base screening of mitochondria with microraft isolation distinguishes pathogenic mitofusin 2 mutations
oleh: Alex L. Yenkin, John C. Bramley, Colin L. Kremitzki, Jason E. Waligorski, Mariel J. Liebeskind, Xinyuan E. Xu, Vinay D. Chandrasekaran, Maria A. Vakaki, Graham W. Bachman, Robi D. Mitra, Jeffrey D. Milbrandt, William J. Buchser
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2022-10-01 |
Deskripsi
Raft-Seq is a generalizable pooled screening platform that combines high-content imaging, machine learning and microraft isolation, and enables efficient screening of genetic perturbations based on their impact on phenotypes.