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Comparing satellite retrieval with high-precision ground observations is an essential component for the validation of CO₂ satellite products. The initial stage of assessing the bias in retrieval products from satellite and ground sources involves establishing a geographical connection between observations that are temporally and spatially proximate. The primary aim of this paper is to evaluate the influence of variations in neighborhood definitions and colocation methods on the assessment of satellite products and provide quantitative references. To achieve this, a series of experiments were conducted involving the Global Total Column Carbon Observation Network (TCCON) and the OCO-2 satellite. Various spatial-temporal neighborhoods and colocation methods were considered in these experiments. The results indicate that spatial neighborhoods exert a more substantial influence on bias compared to temporal neighborhoods. In the mid-latitudes of the Northern Hemisphere, there is an observed linear increase trend between the difference of OCO-2 and TCCON observations and the spatial neighborhood, with an average increase of 0.32 ppm as the neighborhood size changes from 1<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mo>°</mo></mrow></semantics></math></inline-formula> to 10<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mo>°</mo></mrow></semantics></math></inline-formula>. Regarding colocation methods, the simple spatiotemporal geographic constraints tend to overlook changes in the atmospheric state to a certain extent. The target geographic constraint method reduces the bias by 2% to 5% by increasing the proportion of OCO-2 observations targeting TCCON while the method of introducing T₇₀₀ potential temperature reduces by 2% to 13% by screening the gradient of CO₂ concentration change. Moreover, an evident correlation exists between the bias and their corresponding latitudes, with a 0.20 ppm increase in bias observed for every 10<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mo>°</mo></mrow></semantics></math></inline-formula> increment in latitudes in the Northern Hemisphere. The bias of TCCON and OCO-2 shows a pronounced seasonal regularity, with the highest in summer. The study also discusses the selection of spatiotemporal matching with low satellite coverage, the bias distribution, and the attribution of bias to the natural wind field.