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Based on the background of “Industry-Academia-Research-Application” teaching integration, the teaching reform of Instrumental Analysis is the main research object, focusing on the reform and exploration from the aspects of teaching mechanism, teaching methodology, resource library construction, teaching team construction, teaching assessment and so on. It studies the construction mechanism of the integration of Industry-Academia-Research-Application of Instrumental Analysis; explores the specific implementation path of the integration, and researches the problems and characteristic innovations of the integration of Industry-Academia-Research and Application in the course of Instrumental Analysis.