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In the present study, the complete mitochondrial DNA sequence of was determined for the first time. The mitochondrial genome of Lebiasina astrigata is a circular molecule of 16,899 bp in size and contains 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and 2 control regions. The base composition is 30.02% for A, 15.34% for G, 27.24% for C, and 27.40% for T. All protein-coding genes started with Met, ND1,ND2, CO2, ATP8, ATP6, CO3, ND4L, ND5, and ND6 ended by TAA as a stop codon, CO1 ended by AGG as a stop codon, ND4 ended by AGA as a stop codon,CO3, Cytb ended by TAG. The lengths of 12S ribosomal RNA and 16S ribosomal RNA are 951 bp and 1695 bp, respectively. The length of control region is 1221 bp, ranging from 15,678 bp to 16899 bp. The complete mitochondrial genome sequence provided here would be useful for further understanding the evolution of ratite and conservation genetics of Lebiasina astrigata.