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A green synthesis route for the production of silver nanoparticles using methanol extract from <em>Solanum</em> <em>xanthocarpum</em> berry (SXE) is reported in the present investigation. Silver nanoparticles (AgNps), having a surface plasmon resonance (SPR) band centered at 406 nm, were synthesized by reacting SXE (as capping as well as reducing agent) with AgNO<sub>3</sub> during a 25 min process at 45 °C. The synthesized AgNps were characterized using UV–Visible spectrophotometry, powdered X-ray diffraction, and transmission electron microscopy (TEM). The results showed that the time of reaction, temperature and volume ratio of SXE to AgNO<sub>3</sub> could accelerate the reduction rate of Ag<sup>+</sup> and affect the AgNps size and shape. The nanoparticles were found to be about 10 nm in size, mono-dispersed in nature, and spherical in shape. <em>In</em> <em>vitro</em> <strong>anti-<em>Helicobacter</em> <em>pylori</em> activity of synthesized </strong>AgNps<strong> was </strong>tested against 34 clinical isolates and two reference strains of <em>Helicobacter</em> <strong><em>pylori</em> </strong>by the agar dilution method and compared with AgNO<sub>3</sub> and four standard drugs, namely amoxicillin (AMX), clarithromycin (CLA), metronidazole (MNZ) and tetracycline (TET), being used in anti-<em>H.</em> <em>pylori</em> therapy. Typical AgNps sample (S1) effectively inhibited the growth of <em>H.</em> <em>pylori</em>, indicating a stronger anti-<em>H.</em> <em>pylori</em> activity than that of AgNO<sub>3</sub> or MNZ, being almost equally potent to TET and less potent than AMX and CLA. AgNps under study were found to be equally efficient against the antibiotic-resistant and antibiotic-susceptible strains of <em>H.</em> <em>pylori</em>. Besides, in the <em>H.</em> <em>pylori</em> urease inhibitory assay, S1 also exhibited a significant inhibition. Lineweaver-Burk plots revealed that the mechanism of inhibition was noncompetitive.