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Cytochrome P450 (<i>CYP74</i>) family members participate in the generation of oxylipins and play essential roles in plant adaptation. However, the metabolic reprogramming mediated by <i>CYP74s</i> under cold stress remains largely unexplored. Herein, we report how cold-triggered <i>OsHPL1</i>, a member of the <i>CYP74</i> family, modulates rice metabolism. Cold stress significantly induced the expression of <i>OsHPL1</i> and the accumulation of OPDA (12-oxo-phytodienoic acid) and jasmonates in the wild-type (WT) plants. The absence of <i>OsHPL1</i> attenuates OPDA accumulation to a low temperature. Then, we performed a widely targeted metabolomics study covering 597 structurally annotated compounds. In the WT and <i>hpl1</i> plants, cold stress remodeled the metabolism of lipids and amino acids. Although the WT and <i>hpl1</i> mutants shared over one hundred cold-affected differentially accumulated metabolites (DAMs), some displayed distinct cold-responding patterns. Furthermore, we identified 114 and 56 cold-responding DAMs, specifically in the WT and <i>hpl1</i> mutants. In conclusion, our work characterized cold-triggered metabolic rewiring and the metabolic role of <i>OsHPL1</i> in rice.