Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines
oleh: Svetlana Soboleva, Ryo Kurita, Fredrik Ek, Hugo Åkerstrand, Rita Silvério-Alves, Roger Olsson, Yukio Nakamura, Kenichi Miharada
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2021-06-01 |
Deskripsi
In an imaging-based screen of >3,300 compounds compounds, Soboleva et al identify HDAC inhibitors as mediators of erythroid cell enucleation. They further show that the erythroid-specific cell membrane protein, SPTA1, is downregulated in HDAC inhibited cells and that restoration of SPTA1 expression using CRISPR-activation partially rescues the fragility of cells, improving enucleation efficiency.