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Sucrose non-fermenting-1 (SNF1)-related protein kinase 2’s (SnRK2s) are plant-specific serine/threonine protein kinases and play crucial roles in the abscisic acid signaling pathway and abiotic stress response. <i>Ammopiptanthus nanus</i> is a relict xerophyte shrub and extremely tolerant of abiotic stresses. Therefore, we performed genome-wide identification of the <i>AnSnRK2</i> genes and analyzed their expression profiles under osmotic stresses including drought and salinity. A total of 11 <i>AnSnRK2</i> genes (<i>AnSnRK2.1-AnSnRK2.11</i>) were identified in the <i>A. nanus</i> genome and were divided into three groups according to the phylogenetic tree. The <i>AnSnRK2.6</i> has seven introns and others have eight introns. All of the AnSnRK2 proteins are highly conserved at the N-terminus and contain similar motif composition. The result of <i>cis</i>-acting element analysis showed that there were abundant hormone- and stress-related <i>cis</i>-elements in the promoter regions of <i>AnSnRK2s</i>. Moreover, the results of quantitative real-time PCR exhibited that the expression of most <i>AnSnRK2</i>s was induced by NaCl and PEG-6000 treatments, but the expression of <i>AnSnRK2.3</i> and <i>AnSnRK2.6</i> was inhibited, suggesting that the <i>AnSnRK2s</i> might play key roles in stress tolerance. The study provides insights into understanding the function of <i>AnSnRK2s</i>.