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The aim of this present study was to investigate the coversion of ß-carotene to vitamin A by measuring plasma vitamin A and ß3-carotene in dairy cows.<p> In this study, a total of 14 Brown-Swiss Cows, in 4-6 years old, supplied by Konya Animal Research Centre, were, used as materials. Animals were diveded into two equal groups. Group1; control group. Group 2; supplemented with ß-carotene (1 gr/day/each cow).<p> Blood was sampled weekly from the animals during the experimental period of 5 weeks.<p> ß-carotene and vitamin A levels in the blood samples were assayed by using HPLC.<p> In group 2, plasma vitamin A and ß-carotene levels were increased statistically significant (P < 0.0,1, P<0.05).<p> As a conclusion, it will be useful to supplement ß-carotene in dairy cows when the plasma ß-carotene and vitamin A are low.