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Pathogenic yeasts <i>Candida albicans</i> and <i>Candida parapsilosis</i> possess a &#223;-type carbonic anhydrase Nce103p, which is involved in CO₂ hydration and signaling. <i>C. albicans</i> lacking Nce103p cannot survive in low CO₂ concentrations, e.g., in atmospheric growth conditions. <i>Candida</i> carbonic anhydrases are orthologous to the <i>Saccharomyces cerevisiae</i> enzyme, which had originally been detected as a substrate of a non-classical export pathway. However, experimental evidence on localization of <i>C. albicans</i> and <i>C. parapsilosis</i> carbonic anhydrases has not been reported to date. Immunogold labeling and electron microscopy used in the present study showed that carbonic anhydrases are localized in the cell wall and plasmatic membrane of both <i>Candida</i> species. This localization was confirmed by Western blot and mass spectrometry analyses of isolated cell wall and plasma membrane fractions. Further analysis of <i>C. albicans</i> and <i>C. parapsilosis</i> subcellular fractions revealed presence of carbonic anhydrases also in the cytosolic and mitochondrial fractions of <i>Candida</i> cells cultivated in shaken liquid cultures, under the atmospheric conditions.