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The adherent-invasive <i>Escherichia coli</i> (AIEC) pathotype has been implicated in the pathogenesis of inflammatory bowel diseases in general and in Crohn’s disease (CD) in particular. AIEC strains are primarily characterized by their ability to adhere to and invade intestinal epithelial cells. However, the genetic and phenotypic features of AIEC isolates vary greatly as a function of the strain’s clonality, host factors, and the gut microenvironment. It is thus essential to identify the determinants of AIEC pathogenicity and understand their role in intestinal epithelial barrier dysfunction and inflammation. We reasoned that soil nematode <i>Caenorhabditis elegans</i> (a simple but powerful model of host-bacterium interactions) could be used to study the virulence of AIEC vs. non- AIEC <i>E. coli</i> strains. Indeed, we found that the colonization of <i>C. elegans</i> (strain N2) by <i>E. coli</i> impacted survival in a strain-specific manner. Moreover, the AIEC strains’ ability to invade cells in vitro was linked to the median lifespan in <i>C. elegans</i> (strain PX627). However, neither the <i>E. coli</i> intrinsic invasiveness (i.e., the fact for an individual strain to be characterized as invasive or not) nor AIEC’s virulence levels (i.e., the intensity of invasion, established in % from the infectious inoculum) in intestinal epithelial cells was correlated with <i>C. elegans</i>’ lifespan in the killing assay. Nevertheless, AIEC longevity of <i>C. elegans</i> might be a relevant model for screening anti-adhesion drugs and anti-invasive probiotics.