A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo
oleh: Ning Zhao, Kouta Kamijo, Philip D. Fox, Haruka Oda, Tatsuya Morisaki, Yuko Sato, Hiroshi Kimura, Timothy J. Stasevich
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2019-07-01 |
Deskripsi
Expression of genetically encoded antibodies for cell labelling is often limited by folding issues. Here, the authors engineer an anti-HA scFv antibody that works in the cellular environment and use it to track mRNA translation dynamics in living cells and to label proteins in live zebrafish embryos.