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The production and consumption of melange yarns are gradually increasing due to natural multi-colours and strong 3D fashionable effects. The raw material and chemical processing infl uence the environmental and textile values of the melange yarn. Organic cotton and regenerated cellulose fibres are basic raw materials for eco-friendly production of yarns. In this study three different regenerated fibres are blended with organic cotton in three different ratios to manufacture the blended melange yarns of two different shade depths. Therefore, cotton-bamboo viscose, cotton-lyocell and cotton-SeaCell™ blended melange yarns with three blend combination 30/70, 50/50 and 70/30 are manufactured. All the dyed and grey components are mixed at the blow room. The aim of the study is to investigate impact of raw material and blend ratio on mechanical and physical characteristics of different shade depth blended melange yarns. Better yarn characteristics with respect to yarn evenness, imperfections, hairiness, tenacity, elongation, coefficient of friction and diameter are achieved for lower shade depth melange yarn. In cotton-rich blended melange yarns, elongation, hairiness, evenness, total imperfections and diameter are noticeably worse for all the combinations. Statistical analysis shows significant effect of blend type, blend ratio and shade depth on the all yarn properties.