Find in Library

Folate profiles and DNA synthesis were studied during the early germinating period in Vigna radiata. Total folate andmethyl folate forms were increased from 4 to 32 hours during germination period studied. Formyl folates, involved in purine synthesis, were raised 30-fold after 26 hours of incubation followed by DNA synthesis, indicatmg that folate synthesis precedes nucleotide synthesis channelising the one-carbon moieties into nucleotide precursors. This is also supported by the observation of folate mediated one-carbon pool incorporation of 2-'4C-Histidine into nucleic acid during this period. The two folate enzymes, folylpolyglutamate hydrolase which was greatly lowered and folylpolyglutamate synthetase which was significantly elevated, might playa crucial role regulating a balanced supply of the required folate precursors during the early germination period of Vigna radiata.