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<strong>Introduction:</strong> Myxobacteria are Gram-negative Deltaproteobacteria with rod-shaped vegetative cells, aerobic organotrophs that exhibit unique group behavior<em>. </em> <strong>Materials and Methods:</strong>The myxobacterial strain Mx18Zk was isolated from intestinal tract of earth worm of GhaemshahrCounty in Mazandaranprovince and the extraction of the fermented broth culture was prepared with organic solvent extraction method. Methanol extract tested for potential antimicrobial activity against11 various human pathogens. Finally the rest of methanol extract fractionated by HPLC and tested again against sensitive pathogens. <strong>Results:</strong> Strain Mx18Zk significantly inhibited growth of<em> Escherichia coli, Staphylococcus aureus</em> and <em>Chromobacterium violaceum, </em>therefore was used for further characterization. Analysis of morphological, biochemical, 16S rRNA gene sequence and phylogenetic tree indicated that this strain with 100 % homology belongs to the genus<em> Corallococcus macrosporus . </em>The methanol extract was subjected to HPLC fractionation against sensitive pathogens. The active extracts studied by LC-MS and its results compared by previously identified myxobacterial secondary metabolites deposited in Myxobase database of HZI. LC/MS analysis resulted in the identification of Myxochromids and unknown Myxovirescins, Myxalamides antibiotics in methanol extract of the strain Mx18Zk. <strong>Discussion and conclusion:</strong> On the basis of results, isolated strain was active against <em>Escherichia coli, Staphylococcus aureus</em> and <em>Chromobacterium violaceum </em>and100 % homology was belong to the genus<em> Corallococcus macrosporus. </em>LC/MS analysis resulted in the identification of Myxochromids and unknown Myxovirescins, Myxalamides antibiotics in methanol extract of the strain Mx18Zk.