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In this paper, the thermal stability of a Bloch Surface Wave (BSW) assisted bio-photonic sensor is investigated. The structural analysis is carried out using the transfer matrix method (TMM). The design comprises a truncated one-dimensional photonic crystal (1D-PhC) structure along with a defective top layer. The structural parameters are optimized to excite a BSW at the top interface for an operating wavelength of 632.8 nm. The mode confinement is confirmed by using wavelength interrogation, angular interrogation and surface electric field profile. Further, the effect of thermal variation on BSW excitation angle and sensitivity is carried out. The analysis shows the average variations in excitation angle and sensitivity of about −0.00096 degree/<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mo>°</mo><mi mathvariant="normal">C</mi></mrow></semantics></math></inline-formula> and 0.01046 (degree/RIU)/<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mo>°</mo><mi mathvariant="normal">C</mi></mrow></semantics></math></inline-formula>, respectively. Additionally, the analysis is also extended towards different lower wavelengths of 400 nm and 550 nm, which provides average variations in the excitation angles of about −0.0027 degree/<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mo>°</mo><mi mathvariant="normal">C</mi></mrow></semantics></math></inline-formula>, and 0.0016 degree/<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mo>°</mo><mi mathvariant="normal">C</mi></mrow></semantics></math></inline-formula>. This shows that the structural sensitivity response is more thermally stable at the lower wavelength range. Thus, showing its potential applications in designing thermally stable bio-photonic sensors.