Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally defined protein complexes
oleh: Isabel Myriam Schopp, Cinthia Claudia Amaya Ramirez, Jerneja Debeljak, Elisa Kreibich, Merle Skribbe, Klemens Wild, Julien Béthune
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2017-06-01 |
Deskripsi
The BioID approaches takes advantage of the promiscuous biotinylation enzyme (BirA*) to identify proteins that closely interact. Here the authors improve the resolution of BioID using a protein fragment complementation approach that allows the assignment of protein-protein interactions to specific complexes within a common interactome.