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Cytochrome P450 monooxygenase (CYP) is one of the three detoxification metabolic enzymes in insects, and is involved in the metabolism and transformation of endogenous substances as well as the activation and degradation of exogenous compounds. This study aims to reveal the molecular mechanism of <i>CYP9E2</i> in <i>Tribolium castaneum</i> in adapting to high-CO₂ stress. By predicting the sequence function of <i>CYP9E2</i>, analyzing the temporal and spatial expression profile of <i>TcCYP9E2</i>, and using RNAi to silence <i>TcCYP9E2</i> combined with a high-CO₂ stress treatment, we measured the carbohydrate content, trehalase activity, and gene expression levels in trehalose metabolism of <i>T. castaneum</i>. A bioinformatics analysis showed that the predicted molecular weight of the protein encoded by <i>TcCYP9E2</i> is 60.15, the theoretical isoelectric point is 8.63, there is no signal peptide, and the protein is hydrophilic. An evolutionary tree analysis showed that <i>TcCYP9E2</i> belongs to the CYP6 family and belongs to the CYP3 group; and the spatiotemporal expression profile results showed that <i>TcCYP9E2</i> was highly expressed in the larvae midgut 48 h after injection of dsCYP9E2, with survival rates decreasing with the increase in CO₂ concentration. Under the condition of 75% CO₂, the contents of glycogen, glucose, ATP, and membrane-bound trehalase decreased significantly after the injection of dsCYP9E2. The expression of <i>TRE-1</i>, <i>TRE-2</i>, and <i>GP</i> in trehalose metabolism and energy pathways was significantly downregulated.