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ABSTRACTUrban Green Spaces (UGSs) can provide social and ecological benefits, and the coordination of these two functions will largely advance human-nature harmony in cities. As such, we proposed a comprehensive framework to evaluate UGSs multifunctionality, i.e. the coupling coordination relationship of ecological connectivity and spatial accessibility of UGSs. Wuhan City in Central China was taken as a case study, and multiple ecosystem service values of green space patches and the disordered use of UGSs multifunctionality were explored. The results demonstrated low-level coupling coordination of UGSs multifunctionality in Wuhan. More than 70% of UGSs were imbalanced, 27.91% of which featured small sizes, low connectivity and accessibility. We suggest that planners should optimize UGSs arrangement to improve species reachability, especially for species with dispersal distance less than 4 km, and to create 15-minute community life circles for residents at the same time. Our findings are expected to support the promotion of UGSs multifunctionality and the balance of urban development and habitat conservation.