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A novel acidophilic, aerobic bacterium strain, MYW30-H2^T, was isolated from a heap of polymetallic mine. Cells of strain MYW30-H2^T were Gram-stain-positive, endospore-forming, motile, and rod-shaped. Strain MYW30-H2^T grew at a temperature range of 30–45 °C (optimum 40 °C) and a pH range of 3.5–6.0 (optimum 4.0) in the presence of 0–0.5% (<i>w</i>/<i>v</i>) NaCl. Strain MYW30-H2^T could grow heterotrophically on yeast extract and glucose, and grow mixotrophically using ferrous iron as an electron donor with yeast extract. Menaquinone-7 (MK-7) was the sole respiratory quinone of the strain. Iso-C_15:0 and anteiso-C_15:0 were the major cellular fatty acids. The 16S rRNA gene sequence analysis showed that MYW30-H2^T was phylogenetically affiliated with the family <i>Alicyclobacillaceae</i>, and the sequence similarity with other <i>Alicyclobacillaceae</i> genera species was below 91.51%. The average amino acid identity value of the strain with its phylogenetically related species was 52.3–62.1%, which fell into the genus boundary range. The DNA G+C content of the strain was 44.2%. Based on physiological and phylogenetic analyses, strain MYW30-H2^T represents a novel species of a new genus of the family <i>Alicyclobacillaceae</i>, for which the name <i>Fodinisporobacter ferrooxydans</i> gen. nov., sp. nov. is proposed. The type strain is MYW30-H2^T (=CGMCC 1.17422^T = KCTC 43278^T).