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We report the first occurrence of the family Triuridaceae in the Atlantic Forest of Paraná state, Brazil. We found Triuris hyalina Miers in a nature reserve and present a description, taxonomic and ecological comments, distribution data, and images. It is a small, mycoheterotrophic plant species. We found it in an area of dense ombrophilous forest in the municipality of Piraquara, eastern Paraná. Our new record represents the first of the family in the state and the southernmost known occurrence of the species. The new occurrence highlights the importance of floristic surveys to better understand the flora of the Atlantic Forest.