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The seeds of twenty-five varieties/cultivars of mungbean were evaluated in respect of proximate assays, chemical composition, and nutritional values with the aim to ensure the suitability of the foodstuff for human consumption. The average fiber content of the tested varieties/cultivars was 4.85±0.04% while potassium (K) ranges from 250 to 938 and phosphorus (P) from 371 to 464 mg 100 g − 1. The tested cultivars contained micronutrients including Zn, Fe and Cu with average value of 30.70, 104.00 and 3.84 mg kg−1, respectively. Alpha (α-), Gamma (γ-) and Sigma (δ-) tocopherols were also present in mungbean seeds with average value of 25, 1962 and 670 mg kg−1. Among toxic heavy metals, average Cd, Cr and Pb were present at 0.22, 3.23 and 1.64 mg kg−1, respectively, indicating some values above the maximum permissible limit set under various rules and regulations. The content of phytic acid varied from 0.45% to 1.22% in these varieties and it was observed that the phytates were existed in negative correlation with Zn content. Aflatoxins AFB1 and AFB2 were 48% and 16% above the maximum permissible limits, respectively, while AFG1 and AFG2 were found to be below the permissible limits in all the tested varieties/cultivars. The results of the study showed the essential minerals and protein, nutritional and anti-nutritional characters of mungbean varieties/cultivars, which are potential health determinants and can be used to characterize food quality in human healthcare.