Reinvestigation of Carbohydrate Specificity of EBCA-1 Monoclonal Antibody Used for the Detection of <i>Candida</i> Mannan
oleh: Vadim B. Krylov, Arsenii S. Solovev, Ilya A. Puchkin, Dmitry V. Yashunsky, Anna V. Antonets, Olga Y. Kutsevalova, Nikolay E. Nifantiev
| Format: | Article |
|---|---|
| Diterbitkan: | MDPI AG 2021-06-01 |
Deskripsi
Monoclonal antibody EBCA-1 is used in the sandwich immune assay for the detection of circulating <i>Candida</i> mannan in blood sera samples for the diagnosis of invasive candidiasis. To reinvestigate carbohydrate specificity of EBCA-1, a panel of biotinylated oligosaccharides structurally related to distinct fragments of <i>Candida</i> mannan were loaded onto a streptavidin-coated plate to form a glycoarray. Its use demonstrated that EBCA-1 recognizes the trisaccharide β-Man-(1→2)-α-Man-(1→2)-α-Man and not homo-α-(1→2)-linked pentamannoside, as was reported previously.