Isomadecassoside, a New Ursane-Type Triterpene Glycoside from <i>Centella asiatica</i> Leaves, Reduces Nitrite Levels in LPS-Stimulated Macrophages
oleh: Giuseppina Chianese, Francesca Masi, Donatella Cicia, Daniele Ciceri, Sabrina Arpini, Mario Falzoni, Ester Pagano, Orazio Taglialatela-Scafati
| Format: | Article |
|---|---|
| Diterbitkan: | MDPI AG 2021-03-01 |
Deskripsi
A madecassoside-rich fraction obtained from the industrial purification of <i>Centella asiatica</i> leaves afforded a new triterpene glycoside, named isomadecassoside (<b>4</b>), characterized by an ursane-type skeleton and migration of the double bond at Δ<sup>20(21)</sup> in ring E. The structure of isomadecassoside was established by means of HR-ESIMS and detailed analysis of 1D and 2D NMR spectra, which allowed a complete NMR assignment. Studies on isolated J774A.1 macrophages stimulated by LPS revealed that isomadecassoside (<b>4</b>) inhibited nitrite production at non-cytotoxic concentrations, thus indicating an anti-inflammatory effect similar to that of madecassoside.