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The β-<span style="font-variant: small-caps;">d</span>-mannopyranoside linkage is found in a number of biological structures, in particular, in the core trisaccharide of <i>N</i>-linked glycoproteins, as well as within the antigenic polysaccharides of <i>Salmonella</i>, yeasts, and glycolipids. The construction of this glycosydic bond by chemical approach is very challenging and requires cumbersome protection and activation steps prior to glycosylation. In this context, β-mannosidase from <i>Cellulomonas fimi</i> (<i>Cf</i>-β-Man) was immobilized for the first time, and it was employed in the synthesis of β-mannosides. <i>Cf</i>-β-Man immobilized on IDA-Co^2+-agarose allows the synthesis of the disaccharide, cyanomethyl β-<span style="font-variant: small-caps;">d</span>-mannopyranosyl-(1→6)-2-acetamido-2-deoxy-1-thio-β-<span style="font-variant: small-caps;">d</span>-glucopyranoside, with a higher conversion compared to the soluble enzyme (20% vs. 5%) after 6 h under best conditions. This explorative work opens new scenarios concerning the design of engineered <i>Cf</i>-β-Man mutants and their immobilization in order to obtain a robust and recyclable biocatalyst for applications in chemoenzymatic glycan synthesis.