Engineering a riboswitch-based genetic platform for the self-directed evolution of acid-tolerant phenotypes
oleh: Hoang Long Pham, Adison Wong, Niying Chua, Wei Suong Teo, Wen Shan Yew, Matthew Wook Chang
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2017-09-01 |
Deskripsi
Cells are exposed to shifts in environmental pH, which direct their metabolism and behavior. Here the authors design pH-sensing riboswitches to create a gene expression program, digitalize the system to respond to a narrow pH range and apply it to evolve host cells with improved tolerance to a variety of organic acids.