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Background and objective: Acetal resin or polyoxymethylene is a flexible thermoplastic radiolucent denture base material. This study aimed to evaluate the addition of barium sulfate BaSO4 nanoparticles on the mechanical and physical properties of acetal resin. Methods: Forty two specimens were prepared from both non-modified and modified acetal resin. The tested specimens were obtained by melt extrusion into the mold equipment followed by injection molding. The radio-opacity of non-modified and modified evaluated, and then some mechanical and physical properties tested. Results: The results of radio-opacity test showed that the radiographic density of BaSO4 3% Acetal resin specimens was at the same level of 2mm thickness of the aluminum stepwedge. Non-significant decrease noted in surface roughness, creep in all strain regions, and compressive strength and modulus of elasticity. Conclusion: It has been concluded that the addition of 3% BaSO4 nanoparticles produced an acceptable radio-opaque Acetal resin denture base material. Although the addition caused some changes in properties, the modified Acetal resin remained as a flexible thermoplastic material.