Real-Time SARS-CoV-2 Genotyping by High-Throughput Multiplex PCR Reveals the Epidemiology of the Variants of Concern in Qatar
oleh: Mohammad R. Hasan, Mahesh K.R. Kalikiri, Faheem Mirza, Sathyavathi Sundararaju, Anju Sharma, Thabisile Xaba, Stephan Lorenz, Hiam Chemaitelly, Reham A. El-Kahlout, Kin Ming Tsui, Hadi M. Yassine, Peter V. Coyle, Abdullatif Al Khal, Roberto Bertollini, Mohamed H. Al Thani, Laith J. Abu-Raddad, Patrick Tang
| Format: | Article |
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| Diterbitkan: | Elsevier 2021-11-01 |
Deskripsi
Complementing whole genome sequencing strategies with high-throughput multiplex RT-qPCR genotyping allows for more comprehensive and real-time tracking of SARS-CoV-2 variants of concern. During the second and third waves of COVID-19 in Qatar, PCR genotyping, combined with Sanger sequencing of un-typeable samples, was employed to describe the epidemiology of the Alpha, Beta and Delta variants. A total of 9792 nasopharyngeal PCR-positive samples collected between April-June 2021 were successfully genotyped, revealing the importation and transmission dynamics of these three variants in Qatar.