Efficient Expression and Processing of Ebola Virus Glycoprotein Induces Morphological Changes in BmN Cells but Cannot Rescue Deficiency of Bombyx Mori Nucleopolyhedrovirus GP64
oleh: Jinshan Huang, Na Liu, Fanbo Xu, Ellen Ayepa, Charles Amanze, Luping Sun, Yaqin Shen, Miao Yang, Shuwen Yang, Xingjia Shen, Bifang Hao
| Format: | Article |
|---|---|
| Diterbitkan: | MDPI AG 2019-11-01 |
Deskripsi
Ebola virus (EBOV) disease outbreaks have resulted in many fatalities, yet no licensed vaccines are available to prevent infection. Recombinant glycoprotein (GP) production may contribute to finding a cure for Ebola virus disease, which is the key candidate protein for vaccine preparation. To explore GP<sub>1,2</sub> expression in BmN cells, EBOV-GP<sub>1,2</sub> with its native signal peptide or the GP64 signal peptide was cloned and transferred into a normal or gp64 null Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid via transposition. The infectivity of the recombinant bacmids was investigated after transfection, expression and localization of EBOV-GP were investigated, and cell morphological changes were analyzed by TEM. The GP64 signal peptide, but not the GP<sub>1,2</sub> native signal peptide, caused GP<sub>1,2</sub> localization to the cell membrane, and the differentially localized GP<sub>1,2</sub> proteins were cleaved into GP<sub>1</sub> and GP<sub>2</sub> fragments in BmN cells. GP<sub>1,2</sub> expression resulted in dramatic morphological changes in BmN cells in the early stage of infection. However, GP<sub>1,2</sub> expression did not rescue GP64 deficiency in BmNPV infection. This study provides a better understanding of GP expression and processing in BmN cells, which may lay a foundation for EBOV-GP expression using the BmNPV baculovirus expression system.