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In the analysis of the fuel like the liquid fuels obtained from the transesterification process of Spirulina microalgae biomass, the physicochemical characteristics correspond to fractions of gasoline, paraffin, and diesel, and to determine the environmental sustainability of its use, combustion tests were carried out on a diesel test engine (1000, 2000, and 3000 rpm) to establish a comparison with commercial neat diesel. The results obtained prove, based on the analysis of emissions and combustion, that this fuel can be a direct replacement for diesel.