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Stylo (<i>Stylosanthes</i> spp.) is an important pasture legume with strong aluminum (Al) resistance. However, the molecular mechanisms underlying its Al tolerance remain fragmentary. Due to the incomplete genome sequence information of stylo, we first conducted full-length transcriptome sequencing for stylo root tips treated with and without Al and identified three <i>Snakin/GASA</i> genes, namely, <i>SgSnakin1</i>, <i>SgSnakin2</i>, and <i>SgSnakin3</i>. Through quantitative RT-PCR, we found that only <i>SgSnakin1</i> was significantly upregulated by Al treatments in stylo root tips. Histochemical localization assays further verified the Al-enhanced expression of <i>SgSnakin1</i> in stylo root tips. Subcellular localization in both tobacco and onion epidermis cells showed that SgSnakin1 localized to the cell wall. Overexpression of <i>SgSnakin1</i> conferred Al tolerance in transgenic Arabidopsis, as reflected by higher relative root growth and cell vitality, as well as lower Al concentration in the roots of transgenic plants. Additionally, overexpression of <i>SgSnakin1</i> increased the activities of SOD and POD and decreased the levels of O₂^·− and H₂O₂ in transgenic Arabidopsis in response to Al stress. These findings indicate that <i>SgSnakin1</i> may function in Al resistance by enhancing the scavenging of reactive oxygen species through the regulation of antioxidant enzyme activities.