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This paper aims to investigate the behavioral intention to adopt blockchain technology (BC) in Pakistan’s operation and supply chain management. A conceptual framework is developed by extending the unified theory of acceptance and use of technology (UTAUT) model, incorporating technology readiness, technology affinity, and trust. The model was empirically examined using 198 responses from an online survey directed by a Pakistan operation and supply chain professionals. The proposed model is tested using Smart PLS. The empirical results suggest that BC adoption is positively influenced by facilitating condition, social influence, effort expectancy, technology readiness, and technology affinity, while performance expectancy and trust negatively influence BC to adopt. This research contributes to the advancement and stimulation of blockchain technology acceptance behavior in supply chains and significant managerial implications that could be more meaningful for developing economies.