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The domestic water buffalo (Bubalus bubalis) isclassified into the swamp and riverine. However, theirhybrids are also found in some parts of Assam (BrahmaputraValley) in North-east India. Swamp buffaloes have a typicalphenotypic appearance, like the shape of horns, small bodysize as compared to riverine breeds, and body-colour, etc.This study characterizes the indigenous non-descript‘Bhangor’ buffalo population from the Tripura state usingkaryotype analyses. Blood samples were collected fromunrelated animals of both sexes phenotypically identifiedas swamp buffaloes from the breeding tract in Unakotidistrict which has >50% of indigenous buffalo populationin Tripura. The blood leukocytes were cultured, terminated,and harvested using conventional karyotype protocol asstandardised in lab to determine the number ofchromosomes present in the metaphase chromosomespreads after 72 h incubation at 37°C with intermittentshaking in the incubator. Chromosomal spread showedpresence of 2N=48 chromosomes, comprised of 23 pairsof autosomes and a pair of sex chromosomes with clearlydistinct size of fourth pair of metacentric chromosome. Andfor the first time, non-descript indigenous buffalo population‘Bhangor’ from Tripura has been characterized usingkaryotypic analysis.