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Objective To explore the teaching effect of 3D printing knee joint model in eight-year osteoarthritis lecture. Methods Eight-year program students in grade 2014 were selected and randomly divided into an experimental group and a control group. 3D printing knee joint model display + animation demonstration + LBL teaching were used in the experimental group, while animation demonstration + LBL teaching were used in the control group. Teaching effect was evaluated through examination scores and teaching quality evaluation. Results The examina-tion scores, teaching quality scores and the excellent rate of teaching quality were all significantly higher in the experiment group than those in the control group (P＜0.05). Conclusions The 3D printing model can enhance the learning enthusiasm and improve students' mastery of knowledge and so is recommended.